Publications

2022

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus,

Developmental Biology

Alice M. Godden, Marco Antonaci, Nicole J. Ward, Michael van der Lee, Anita Abu-Daya, Matthew Guille, Grant N. Wheeler

2021

Characterising open chromatin in chick embryos identifies cis-regulatory elements important for paraxial mesoderm formation and axis extension

• Gi Fay Mok, 
• Leighton Folkes, 
• Shannon A. Weldon, 
• Eirini Maniou, 
• Victor Martinez-Heredia, 
• Alice M. Godden, 
• Ruth M. Williams, 
• Tatjana Sauka-Spengler, 
• Grant N. Wheeler, 
• Simon Moxon & 
• Andrea E. Münsterberg 

2020

In Vivo Assessment of Drug-Induced Hepatotoxicity Using Xenopus Embryos.

Saide K, Wheeler GN.Cold Spring Harb Protoc. 2020 Nov 2;2020(11). doi: 10.1101/pdb.prot106096.

 

Microsyntenic Clusters Reveal Conservation of lncRNAs in Chordates Despite Absence of Sequence Conservation.

Herrera-Úbeda C, Marín-Barba M, Navas-Pérez E, Gravemeyer J, Albuixech-Crespo B, Wheeler GN, Garcia-Fernàndez J.Biology (Basel). 2019 Aug 24;8(3):61. doi: 10.3390/biology8030061.

FZD10 regulates cell proliferation and mediates Wnt1 induced neurogenesis in the developing spinal cord Alrefaei AF, Münsterberg AE, Wheeler GN. PLoS One. 2020 Jun 12;15(6):e0219721. doi: 10.1371/journal.pone.0219721. eCollection 2020. 

2019

Solid lipid nanoparticles for the delivery of anti-microbial oligonucleotides. González-Paredes A, Sitia L, Ruyra A, Morris CJ, Wheeler GN, McArthur M, Gasco P.

Eur J Pharm Biopharm. 2019 Jan;134:166-177. doi: 10.1016/j.ejpb.2018.11.017. Epub 2018 Nov 20.

2018

microRNAs associated with early neural crest development in Xenopus laevis.

Ward NJ, Green D, Higgins J, Dalmay T, Münsterberg A, Moxon S, Wheeler GN.BMC Genomics. 2018 Jan 18;19(1):59. doi: 10.1186/s12864-018-4436-0.

Paracetamol-induced liver injury modelled in Xenopus laevis embryos.Saide K, Sherwood V, Wheeler GN.Toxicol Lett. 2019 Mar 1;302:83-91. doi: 10.1016/j.toxlet.2018.09.016. Epub 2018 Sep 30

ADAMTS9, a member of the ADAMTS family, in Xenopus development.

Desanlis I, Felstead HL, Edwards DR, Wheeler GN.Gene Expr Patterns. 2018 Sep;29:72-81. doi: 10.1016/j.gep.2018.06.001. Epub 2018 Jun 21.

   Gi Fay Mok, Estefania Lozano-Velasco, Eirini Maniou, Camille Viaut, Simon Moxon, Grant N. Wheeler, and Andrea Erika Munsterberg (2018). miR-133 mediated regulation of the Hedgehog pathway orchestrates embryo myogenesis. Development, in press 

      Nicole J Ward, Darrell Green, Janet Higgins, Tamas Dalmay, Andrea Münsterberg, Simon Moxon and Grant N Wheeler (2018). microRNAs associated with early neural crest development in Xenopus laevis. BMC Genomics,

      Marta Marín-Barba, Helena Gavilán, Lucia Gutierrez, Estefanía Lozano-Velasco, Ildefonso Rodriguez-Ramiro, Grant N. Wheeler, Christopher Morris, Puerto M. Morales, Amalia Ruiz, (2018). Unravelling the mechanisms that determine the uptake and metabolism of magnetic single and multicore nanoparticles in a Xenopus laevis model. Nanoscale, 10(2):690-704

2017

   Kimberley Hanson, Stephen R. Robinson, Karamallah Al-Yousuf, Adam E. Hendry, Darren W. Sexton, Victoria Sherwood and Grant N. Wheeler (2017). The anti-rheumatic drug, leflunomide, synergizes with MEK inhibition to suppress melanoma growth. Oncotarget, 9(3):3815-3829

      Muhammad Abu-Elmagd, Joanna Mulvaney and Grant N. Wheeler (2017). Frizzled-7 is required for Xenopus heart development. Biology Open, 6(12):1861-1868

      Ruth M Williams, Robert J Winkfein, Rebecca S Ginger, Martin R Green, Paul P Schnetkamp and Grant N Wheeler (2017). A functional approach to understanding the role of NCKX5 in Xenopus pigmentation. Plos One, 12(7):e0180465

Marianna Mamusa, Leopoldo Sitia, Francesco Barbero, Angels Ruyra, Teresa Diaz Calvo, Costanza Montis, Ana Gonzalez-Paredes, Grant N Wheeler, Christopher J Morris, Michael McArthur and Debora Berti (2017). Cationic liposomal vectors incorporating a bolaamphiphile for oligonucleotide antimicrobials. BBA – Biomembranes, 1859(10):1767-1777

       Karamallah Al-Yousuf, Carl A. Webster, Grant N. Wheeler, Francesca Baldelli Bombelli and Victoria Sherwood (2017). Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety. Current Protocols in Toxicology, 73:20.13.1-20.

2016

   Victoria L. Hatch, Marta Marin-Barba, Simon Moxon, Christopher T. Ford, Nicole J. Ward, Matthew L. Tomlinson, Ines Desanlis, Adam E. Hendry, Saartje Hontelez, Ila van Kruijsbergen, Gert Jan C. Veenstra, Andrea E. Münsterberg and Grant N. Wheeler (2016). The Positive Transcriptional Elongation Factor (P-TEFb) is required for Neural Crest Specification. Developmental Biology, 416: 361-372

   Webster, C. A., Di Silvio, D., Devarajan, A., Bigini, P., Micotti, E., Giudice, C., Salmona, M., Wheeler, G. N., Sherwood, V.* and Baldelli Bombelli, F.* (2016) An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian nanoparticle toxicity assessment. Nanomedicine, 11(6):643-56 [*equal contribution].

2015

   Ayisha Ahmed, Nicole J. Ward, Simon Moxon, Sara Lopez-Gomollon, Camille Viaut, Matthew L. Tomlinson, Ilya Patrushev, Michael J. Gilchrist, Tamas Dalmay, Dario Dotlic, Andrea E. Münsterberg and Grant N. Wheeler (2015). A database of microRNA expression patterns in Xenopus laevis. PLoS ONE, 10(10): e0138313.

   Hoppler S and Wheeler GN. (2015) It's about time for neural crest. Science, 348 (6241):1316-1317

   Kelwick R, Desanlis I, Wheeler GN, Edwards DR. (2015) The ADAMTS (A Disintegrin and Metalloproteinase with Thrombospondin motifs) family. Genome Biol. 16(1):113

   Alaa Abou-Elhamd, Abdulmajeed Alrefaei, Gi Fay Mok, Carla Garcia-Morales, Muhammad Abu-Elmagd, Grant N. Wheeler and Andrea E. Münsterberg (2015). Klhl31 attenuates -catenin dependent Wnt signaling and regulates embryo myogenesis. Developmental Biology, 402(1):61-71.

   Muhammad Abu-Elmagd, Katarzyna Goljanek‑Whysall, Grant Wheeler and Andrea Münsterberg (2015). Sprouty2 mediated tuning of signalling is essential for somite myogenesis, BMC Medical Genomics. 8(Suppl 1):S8

2014

   Katarzyna Goljanek-Whysall, Gi Fay Mok, Abdulmajeed Alrefaei, Niki Kennerley, Grant Wheeler and Andrea Münsterberg (2014). myomiR dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis, Development 141(17):3378-87.

   Junfang Song, James McColl, Esther Camp, Niki Kennerley , Dominique McCormick, Tim Grocott , Grant N. Wheeler and Andrea Münsterberg (2014). Smad1 integrates BMP2 and Wnt3a signals in migrating cardiac progenitor cells. PNAS. 111 (20) 7337-7342

   Georgios Drakakis, Adam E. Hendry, Kimberley Hanson, Suzanne C. Brewerton, Michael J. Bodkin, David A. Evans, Grant N. Wheeler and Andreas Bender (2014). Bioactivity Profile Analysis from Automated Phenotype Detection in Xenopus laevis Using Image Processing. Medicinal Chemistry Communications. 5:386-396

2013

   Sonia Liggi, Georgios Drakakis, Adam E. Hendry, Kimberley M. Hanson, Suzanne C. Brewerton, Grant N. Wheeler, Michael J. Bodkin, David A. Evans, Andreas Bender (2013). Extensions to In Silico Bioactivity Predictions Using Pathway Annotations and Differential Pharmacology Analysis: Application to Xenopus laevis Phenotypic Readouts. Molecular Informatics, 32:1009-1024

2012

   Matthew L. Tomlinson, Adam Hendry and Grant N. Wheeler (2012). Chemical genetics and drug discovery in Xenopus. Methods Mol Biol. 917:155-166

   Grant N. Wheeler and Karen J. Liu (2012). Xenopus: an ideal system for chemical genetics. Genesis. 50(3):207-18.

   Wheeler, G.N., Field, R.A., and M.L. Tomlinson  (2012). Phenotypic screens with model organisms. In H. Fu (Ed), Chemical Genomics (pp. 121-136). New York: Cambridge University Press.

2011

   White RM, Cech J, Ratanasirintrawoot S, Lin CY, Rahl PB, Burke CJ, Langdon E, Tomlinson ML, Mosher J, Kaufman C, Chen F, Long HK, Kramer M, Datta S, Neuberg D, Granter S, Young RA, Morrison S, Wheeler GN, Zon LI. (2011) DHODH modulates transcriptional elongation in the neural crest and melanoma. Nature. 471:518-22

This paper generated a large amount of press interest upon publication. GNW was interviewed on TV by Sky News, BBC and Anglia TV. Radio Norfolk. Articles appeared in The Daily Telegraph, Daily Mirror, Metro, i, Western Daily Press, EDP, Evening News, BBC Online, Manchester Evening News, Reuters and numerous others including many websites and blogs.

2009

 Carla Garcia-Morales, Chiung-Hui Liu, Muhammad Abu-Elmagd, Mohammad Hajihosseini and Grant N. Wheeler (2009). Frizzled 10 promotes sensory neuron development in Xenopus embryos. Developmental Biology, 335:143-155

  Grant N. Wheeler and Andre Brandlii. Simple vertebrate models for chemical genetics and drug discovery screens: lessons from zebrafish and Xenopus. (2009) Dev Dyn. 238:1287-1308.

27.   Matthew L. Tomlinson, Martin Rejzek, Mark Fidock, Robert A. Field and Grant N. Wheeler. (2009) Chemical genomics identifies compounds affecting Xenopus laevis pigment cell development. Molecular BioSystems, 5:376-384

   Matthew L. Tomlinson, Pingping Guan, Richard J. Morris, Mark Fidock, Martin Rejzek, Carla Garcia-Morales, Robert A. Field and Grant N. Wheeler. (2009) A chemical genomic approach identifies matrix metalloproteinases as playing an essential and specific role in Xenopus melanophore migration. Chemistry and Biology, 16:1-12

This paper generated a lot of press interest upon publication. Articles appeared in The Daily Telegraph, Daily Mirror, Metro, Liverpool Daily Post, The Northern Echo, Western Daily Press, EDP, Evening News, BBC Online (http://news.bbc.co.uk/1/hi/health/7851932.stm), Manchester Evening News, Reuters and numerous others including many websites and blogs. GNW was also interviewed on RTE Radio (Dublin).

2008

   Wheeler G. N., Lavery D. and Hoppler S., (2008). Inducible gene expression in transient transgenic Xenopus embryos. Methods Mol Biol. 469:431-49.

   Matthew Tomlinson, Muhammad Abu-Elmagd, Carla Garcia-Morales and Grant N. Wheeler. A combinatorial role for three matrix metalloproteinases in Xenopus macrophage migration (2008). Mechanism of Development, 125:1059-1070

   Danielle L. Lavery, Ian R. Davenport, Grant N. Wheeler and Stefan Hoppler (2008). Wnt6 expression in epidermis and epithelial tissues during Xenopus organogenesis. Developmental Dynamics 237(3):768-79

2007

   Grocott T., Maillard M., Jonassen J., Wheeler G. N., Dawes L., Wormstone M. and Chantry A. (2007). The MH1 Domain of SMAD3 interacts with Pax6 and represses the autoregulation of Pax6 transcriptional activity. Nucleic Acids Research, 35(3):890-901

2006

   Muhammad Abu-Elmagd, Carla Garcia-Morales and Grant N. Wheeler (2006). Frizzled 7 mediates canonical Wnt signalling in neural crest induction. Developmental Biology, 298:285-298

   Münsterberg, A. and Wheeler, G. (2006). Cell movements during early vertebrate morphogenesis., In: Unsicker, K., Krieglstein, K. (Eds.): Cell Signaling and Growth Factors in Development. Volume 1, 107-140, Wiley-VCH, Weinheim

   Dylan Sweetman, Tina Rathjen, Matthew Jefferson, Guy Wheeler, Terence G. Smith, Grant N. Wheeler, Andrea Munsterberg and Tamas Dalmay. (2006). FGF-4 regulates mir-206 expression in developing somites of chicken embryos. Developmental Dynamics, 235:2185-2191

2005

   Matthew Tomlinson, Rob Field and Grant N. Wheeler (2005). Xenopus as a Model Organism in Developmental Chemical Genetic Screens. Molecular BioSystems 1:223-228

(This paper was chosen as a Hot Paper by the journal and was highlighted in an issue of Chemical World)

2004

  Michael Harrison, Muhammad Abu-Elmagd, Timothy Grocott, Clara Yates, Jelena Gavrilovic and Grant N. Wheeler (2004). Matrix Metalloproteinases in Xenopus Development. Developmental Dynamics 231:214-220

  Andrea Münsterberg, Qiaoyun Yue, Muhammad Abu-Elmagd, Laura Wagstaff and Grant Wheeler (2004). The molecular mechanisms controlling cardiac precursor cell specification and movements. The Bulletin of the British Society for Cardiovascular Research, 17:5-10

2001

   Grant N. Wheeler and Richard O. Hynes (2001) The Cloning, genomic organisation and expression of the focal contact protein Paxillin in Drosophila. Gene 262:291-299

   Fiona S. Hamilton, Grant N. Wheeler and Stefan Hoppler (2001). Difference in XTcf-3 dependency accounts for change in response to -catenin-mediated Wnt signalling in Xenopus blastula. Development 128:2063-2073

2000

   Grant N. Wheeler, Fiona Hamilton and Stefan Hoppler (2000) Inducible Gene Expression in Transgenic Xenopus Embryos. Current Biology 10:849-82

1999

   Grant N. Wheeler and Stefan Hoppler (1999) Two novel Xenopus frizzled genes expressed in developing heart and brain. Mechanisms of Development 86:203-207

   Dirk Geerts, Lionel Fontao, Mirjam G. Nievers, Ingrid Kuikman, Roel Q. J. Schaapveld, Trisha Purkis, Grant Wheeler, E. Birgitte Lane, Irene M. Leigh and Arnoud Sonnenberg (1999) Binding of the integrin 64 to plectin prevents its association with actin stress fibres but not that with intermediate filaments. J. Cell Biol. 147:417-434

1994

   Roger S. Buxton, Grant N. Wheeler, Sara C. Pidsley, Mark D. Marsden, Michael J. Adams, Nancy A. Jenkins, Debra J. Gilbert, and Neal G. Copeland (1994). Mouse Desmocollin (Dsc3) and Desmoglein (Dsg1) genes are closely linked in the proximal region of chromosome 18. Genomics 21:510-516.

1991

  Grant N. Wheeler, Roger S. Buxton, Andrew E. Parker, Joachim Arnemann, David A. Rees, Ian A. King and Anthony I. Magee (1991). Desmosomal cadherins I, II and III: novel members of the cadherin superfamily. Biochem. Soc. Trans. 19:1060-1064

   Andrew E. Parker, Grant N. Wheeler, Joachim Arnemann, Sara C. Pidsley, Paris Ataliotis, Claire L. Thomas, David A. Rees, Anthony I. Magee and Roger S. Buxton (1991). Desmosomal Glycoproteins II and III: Cadherin-like Junctional Molecules generated by Alternative Splicing. J. Biol. Chem. 266:10438-10445

   Arnemann J, Spurr N.K., Wheeler G.N., Parker A.E., Buxton R.S. (1991). Chromosomal assignment of the human genes coding for the major proteins of the desmosome junction, desmoglein DGI (DSG), desmocollins DGII/III (DSC), desmoplakins DPI/II (DSP), and plakoglobin DPIII (JUP). Genomics. 10(3):640-5.

   G.N. Wheeler, A.E. Parker, C.L. Thomas, P. Ataliotis, D. Poynter, J. Arnemann, A.J. Rutman, S.C. Pidsley, F.M. Watt, D.A. Rees, R.S. Buxton and A.I. Magee (1991). Desmosomal Glycoprotein I, A Component of Intercellular Desmosome Junctions, is Related to the Cadherin Family of Cell Adhesion Molecules. Proc. Nat. Acad. Sci. USA 88:4796-4800.

1990

   J.C. Brown, J.H. Spragg, G.N. Wheeler and P.W. Taylor (1990). Identification of the B1 and B2 subunits of human placental laminin and rat parietal-yolk-sac laminin using antisera specific for murine laminin-ß-galactosidase fusion proteins. Biochem. J. 270:463-468.